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Gel Permeation Chromatography (GPC), Size Exclusion Chromatography (SEC), or Gel Filtration Chromatography (GFC) are names used interchangeably for a liquid chromatography technique to obtain separation according to molecular dimensions. Contrary to LAC- (Liquid Adsorption Chromatography) methods like HPLC, that rely on interactions between sample and stationary phase (column material), GPC/SEC must operate free of interactions. Only then is a separation according the size of the molecules ensured. Ideally, only entropic effects should influence this type of separation. |
